Next-generation sequencing (NGS) coverage describes the average number of reads that align to, or "cover," known reference bases. In order words, it counts the number of reads getting aligned to a particular nucleotide base position or coordinate. The sequencing coverage level often determines whether variant discovery can be made with a certain degree of confidence at particular base positions. KNOW MORE

NGS reads are aligned optimally to a standard reference sequence. The alignment gives the read count value of each coordinate of the DNA. If a coordinate has c read count value, i.e., c number of reads got aligned to that coordinate, then out of those c reads Quantification gives us the number of reads, whose base got exact match with the reference base.

Copy number variations (CNVs) are structural variants consisting of large-scale insertions and deletions of genomic fragments. Exploring CNVs and estimating their effects on phenotypes are useful for genome selection but remain challenging in the livestock. KNOW MORE